- 貨號 Cat. No.
- ANT-006-AG
- 規格 Size
- 50 µl
Description
Anti-proBDNF-ATTO-488
Epitope: |
Peptide(C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560 ). |
Putative epitope location: |
Prodomain of the BDNF protein.
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Homology: |
Mouse, rat 16 /17 amino acid residues identical.
Label:
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
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Reactivity Confirmed: |
Human, rat, mouse.
Specificity:
Standard Quality Control of each lot: |
Western blot analysis (unlabeled antibody, see datasheet of product (#ANT-006) and immunohistochemistry (labeled antibody).
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Applications:
Immunohistochemistry: |
Expression of proBDNF in rat DRG |
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Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-proBDNF-ATTO-488antibody (#ANT-006-AG), (1:50), (green). Staining is present in DRG neurons.Hoechst 33342 is used as the counterstain (blue).
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Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).
All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.
Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.
The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP) a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimerî–¸ disease.
Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. ProBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.
Alomone Labs offers a highly specific antibody directed against an epitope located in the prodomain region of human BDNF.Anti-proBDNF antibody (#ANT-006) does not cross react with mature BDNF, pro and mature NGF or mature NT-3. Anti-proBDNF can be used in western blot, and immunohistochemical applications. It has been designed to recognize proBDNF from human, rat and mouse samples.
Alomone Labs is offering a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-proBDNF-ATTO-448 antibody (#ANT-006-AG) has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.
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