Kits
anti- PR-3 (c-ANCA)
Kits
Description
Anti-neutrophilic-cytoplasm antibodies (ANCA) represents
a group of autoantibodies directed towards the
cytoplasmatic components of the neutrophilic granulocytes
and monocytes. The classical methods for the
determination of ANCA are the immunofluorescent
methods. With these indirect immunofluorescence
techniques two main patterns are recognized, a
cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.
The main antigen for the c-ANCA is the proteinase 3 (PR3),
which is a serine proteinase present in primary
granules. Antibodies of p-ANCA positive sera are mainly
directed to myeloperoxidase (MPO). Antibodies to other
antigens e.g. lactoferrin, elastase, cathepsin-G and also
lysozyme often result in a similar p-ANCA pattern. Beside
different untypical variants of p-ANCA IF patterns
granulocyte specific antinuclear antibodies (GS-ANA) is
indistinguishable from p-ANCA. This makes a clear
interpretation and classification of the IF patterns difficult.
Therefore every positive IF-ANCA findings esp. p-ANCA
should be differenciated by ELISA techniques using
purified antigens. A survey of documented clinical
indications of specific ANCA is given in the table below.
PR-3- ANCA and MPO-ANCA are reliable serologic
markers in the diagnostics of vasculitides.
PR-3- ANCA is the classical autoantigen in Wegener´s
granulomatosis with a clinical specificity of more than 95%.
c-ANCA is documented to be present in different diseases.
Anti-MPO antibodies are highly specific for idiopathic and
vasculitis associated crescentic glomerulonephritis and
also for classic polyarteritis nodosa, Churg-Strauss
syndrome and the polyangitis overlap syndrome without
renal involvement. With respect to sensitivity, either MPO
or PR-3 antibodies were found in 77 to 100% of patients
with idiopathic and vasculitis associated crescentic
glomerulonephritis. In WG, anti-MPO antibodies were
detected only occasionally and generally in patients
negative for PR-3 antibodies.
The MPO and PR-3 specific ELISA methods can provide
an important confirmatory result for two of the more
important of the identified antigens. ELISA is also useful for
interpreting difficult samples by IFA such as those which
exhibit several antibodies simultaneously or those with high
background fluorescence.
a group of autoantibodies directed towards the
cytoplasmatic components of the neutrophilic granulocytes
and monocytes. The classical methods for the
determination of ANCA are the immunofluorescent
methods. With these indirect immunofluorescence
techniques two main patterns are recognized, a
cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.
The main antigen for the c-ANCA is the proteinase 3 (PR3),
which is a serine proteinase present in primary
granules. Antibodies of p-ANCA positive sera are mainly
directed to myeloperoxidase (MPO). Antibodies to other
antigens e.g. lactoferrin, elastase, cathepsin-G and also
lysozyme often result in a similar p-ANCA pattern. Beside
different untypical variants of p-ANCA IF patterns
granulocyte specific antinuclear antibodies (GS-ANA) is
indistinguishable from p-ANCA. This makes a clear
interpretation and classification of the IF patterns difficult.
Therefore every positive IF-ANCA findings esp. p-ANCA
should be differenciated by ELISA techniques using
purified antigens. A survey of documented clinical
indications of specific ANCA is given in the table below.
PR-3- ANCA and MPO-ANCA are reliable serologic
markers in the diagnostics of vasculitides.
PR-3- ANCA is the classical autoantigen in Wegener´s
granulomatosis with a clinical specificity of more than 95%.
c-ANCA is documented to be present in different diseases.
Anti-MPO antibodies are highly specific for idiopathic and
vasculitis associated crescentic glomerulonephritis and
also for classic polyarteritis nodosa, Churg-Strauss
syndrome and the polyangitis overlap syndrome without
renal involvement. With respect to sensitivity, either MPO
or PR-3 antibodies were found in 77 to 100% of patients
with idiopathic and vasculitis associated crescentic
glomerulonephritis. In WG, anti-MPO antibodies were
detected only occasionally and generally in patients
negative for PR-3 antibodies.
The MPO and PR-3 specific ELISA methods can provide
an important confirmatory result for two of the more
important of the identified antigens. ELISA is also useful for
interpreting difficult samples by IFA such as those which
exhibit several antibodies simultaneously or those with high
background fluorescence.