Anti-PGLS Antibody

Anti-PGLS Antibody LS-B4026

PGLS Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4026 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4026 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500. Learn more

PGLS Antibody Details

Target: Human PGLS

Host: Rabbit

Reactivity: Human

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

PGLS Antibody Specificity

Immunogen: PGLS antibody was raised against synthetic peptide from internal region of human PGLS (NP_036220.1, O95336). Percent identity by BLAST analysis: Dog (100%); Mouse, Rat, Guinea pig (92%).

Epitope: Internal

Specificity: Human PGLS

PGLS Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.