Anti-Pembrolizumab Neutralizing Antibody ELISA kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
- Application 應用
- Used for the quantitative determination of Anti-Pembrolizumab Neutralizing Antibody concentration in serum and plasma.
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative competitive enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD279 antigen and then pipetted into the wells. Anti-Pembrolizumab Neutralizing Antibody in the sample competitively binds to Pembrolizumab with the biotin-labeled Human CD279 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pembrolizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative competitive enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD279 antigen and then pipetted into the wells. Anti-Pembrolizumab Neutralizing Antibody in the sample competitively binds to Pembrolizumab with the biotin-labeled Human CD279 antigen. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Pembrolizumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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