Anti-PCNA Antibody

Anti-PCNA Antibody LS-B3434

PCNA Mouse anti-Rat Monoclonal (PC10) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3434 was determined to be 10 ug/ml. Learn more

PCNA Antibody Details

 

 

Target:

Rat PCNA

 

Host:

Mouse

 

Reactivity:

Chicken, Drosophila, Human, Mouse, Rat, Yeast

 

Clonality:

 Monoclonal [PC10]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry (IHC) (10 µg/ml)

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB) (1:5000)

 

Immunoprecipitation (IP)

 

Optimal dilution to be determined by the researcher.

 

PCNA Antibody Specificity

 

Immunogen:

PCNA antibody was raised against proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein

 

Specificity:

Specific for PCNA p36 protein expressed at high levels in proliferating cells.

PCNA Antibody Packaging

 

Presentation:

Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.