Anti-PARP Antibody

Anti-PARP Antibody LS-B3432

PARP Mouse anti-Human Monoclonal (A6.4.12) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3432 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3432 was determined to be 10 ug/ml. Learn more

PARP Antibody Details

 

 

Target:

Human PARP

 

Host:

Mouse

 

Reactivity:

Drosophila, Xenopus, Hamster, Human, Mouse, Rat

 

Clonality:

IgG1 Monoclonal [A6.4.12]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Western blot (WB)

 

Immunoprecipitation (IP)

 

ELISA (1:1000 - 1:5000)

 

Optimal dilution to be determined by the researcher.

 

PARP Antibody Specificity

 

Immunogen:

PARP1 antibody was raised against human PARP.

 

Specificity:

Recognizes poly (ADP-ribose) polymerase (PARP), a 116kD nuclear enzyme that has been shown to be cleaved during apoptosis

PARP Antibody Packaging

 

Presentation:

0.09% sodium azide.

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.