Anti-P4HA1 Antibody

Anti-P4HA1 Antibody LS-B3291

P4HA1 Goat anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3291 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3291 was determined to be 5 ug/ml. Learn more

P4HA1 Antibody Details

 

 

Target:

Human P4HA1

 

Host:

Goat

 

Reactivity:

Dog, Human, Mouse, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB) (1:32000) & (0.1 µg/ml)

 

ELISA (1:32000)

 

Optimal dilution to be determined by the researcher.

 

P4HA1 Antibody Specificity

 

Immunogen:

P4HA1 antibody was raised against synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2; NP_001017962.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Orangutan (100%), Gibbon (100%), Marmoset (100%), Dog (100%), Bat (100%), Bovine (100%), Horse (100%), Rabbit (100%), Pig (100%), Turkey (100%), Chicken (100%), Platypus (100%), Lizard (100%), Mouse (92%), Rat (92%), Hamster (92%), Opossum (92%), Salmon (92%), Pufferfish (92%), Zebrafish (92%).

 

Epitope:

Internal

 

Specificity:

Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

P4HA1 Antibody Packaging

 

Presentation:

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

 

Storage:

Store at -20°C. Minimize freezing and thawing.

 

Restrictions:

For research use only.