Anti-P2Y12
- 貨號 Cat. No.
- APR-012
- 規格 Size
- 50 μl 0.2ml
Description
Anti-P2Y12
| Product#: | APR-012 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit
|
| Epitope: |
Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2Y12 receptor. |
 |
| Putative epitope location: |
Cytoplasmic. |
| Homology: |
Rat, mouse - 16/18 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, human, mouse. |
Applications:
| Western Blotting: |
Rat brain membranes, human platelets (1:200). |
 | Western blotting of rat brain (1,2) membranes or human platelets (3,4) 1,3. Anti-P2Y12 antibody (#APR-012), (1:200). 2,4. Anti-P2Y12 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of P2Y12 in mouse brain |
 |
Immunohistochemical staining of P2Y12 (red) in mouse brain using Anti-P2Y12 antibody (#APR-012). Counterstaining with sytox green (green). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. |
| References: |
| 1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. |
| 2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. |
| 3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. |
| 4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416. |
The P2Y receptors belong to the G-protein coupled receptors superfamily.
They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP AND UDPA. Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4
They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP AND UDPA. Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3
The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human P2Y12 receptor. The Anti-P2Y12 antibody (#APR-012) can be used for Western Blotting and Immunohistochemical applications. It has been designed to recognize P2Y12 receptor from human, rat and mouse samples.