Anti-P2Y11
- 貨號 Cat. No.
- APR-015
- 規格 Size
- 50 μl 0.2ml
Description
Anti-P2Y11
| Product#: | APR-015 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)NATAAPKPSEPQSRELS corresponding to residues 357-373 of human P2Y11(Accession Q96G91). |
| Putative epitope location: |
C-Terminus, Intracellular. |
 |
| Homology: |
Human, rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat spleen (lanes 1,4), rat lung (lanes 2,5) and human platelets (lanes 3,6) lysates: 1-3. Anti-P2Y11 antibody (#APR-015) (1:200). 4-6. Anti-P2Y11 antibody, preincubated with the control peptide antigen. |
 | Western blotting of RAEC (lanes 7,9) and ms1 (lanes 8,10) cell line lysates: 7-8. Anti-P2Y11 antibody (#APR-015) (1:200). 9-10. Anti-P2Y11 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Staining of P2Y11 receptor in rat spleen using Anti-P2Y11 antibody(#APR-015). Secondary (activated) follicle in the spleen white pulp shows intense staining of Marginal Zone T lymphocytes (red arrow); note that cells in the red pulp (green arrow) and B lymphocytes in the germinal center (blue arrow) are not stained. Yellow arrows show mononuclear cells with intense stain, probably non specific due to very high intrinsic peroxidase activity. DAB product is brown and the counterstain is cresyl violet. The two images are the same at different augmentation (Left pane 200x and right pane 400x). |
| Immunocytochemistry: |
Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells |
 |
Immunocytochemical staining of P2Y11 inhumanP2Y11-MDCK transfected cells. Paraformaldehyde-fixed and permeabilized humanP2Y11-MDCK transfected cell were stained as described. A. Immunocytochemical staining using Anti-P2Y11 antibody (#APR-015), (1:200) followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. |
| References: |
| 1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. |
| 2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. |
| 3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. |
| 4. Communi, D. et al. (2000) FEBS Letters 475, 39. |
| 5. White, P.J. et al. (2003) Mol. Pharmacol. 63, 1356. |
| 6. Wang, L. et al. (2002) J.Cardiol. Pharmacol. 40, 841. |
| 7. http://www.iuphar-db.org/iuphar-rd/query?type=link&lType=chapter&id=1294. |
The P2Y receptors belong to the G-protein coupled receptors superfamily.
They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP
and UDP). Eight functional mammalian P2Y receptors have been described:
P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor,
now renamed P2Y14.1-3
They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP
and UDP). Eight functional mammalian P2Y receptors have been described:
P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor,
now renamed P2Y14.1-3
The human P2Y11 receptor is unique among the P2Y receptors since it is dually
coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly
expressed in spleen and for much lower extent at platelets.
Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes
(HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4,7
coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly
expressed in spleen and for much lower extent at platelets.
Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes
(HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4,7