Anti-P2X7 (extracellular)-FITC

Anti-P2X7 (extracellular)-FITC

(P2Z)

Product#:

APR-008-F

Sizes:

50 ml

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP.  

To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.  

The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the  

central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system,  

particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release  

of proinflamatory cytokines, stimulation of transcription factors and may also have an important  

role in apoptosis.^1-3 Different techniques have been used to characterize the P2X7 receptor.  

Most of them investigated pores, ion channels (electrophysiology) and membrane alterations  

(calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis).  

With the introduction of flow cytometry, it is now possible to analyze multiple cell  

parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.⁴ 

There is an increasing need for extracellular antibodies that will enable the use of flow cytometry  

for exploring the distribution and expression pattern of the P2X7 receptor.  

Host:

Rabbit.

Epitope:

Peptide KKGWMDPQSKGIQTGRC, corresponding to residues 136-152 of mouse P2X7 receptor.

Putative epitope location:

Extracellular.

Homology:

Human, rat?identical; bovine- 14/17 residues identical. Label:   Fluorescein isothiocyanate (FITC)

Reactivity Confirmed:

Human.

Applications:

Immunocytochemistry:

Immunocytochemistry of K562 living cells stained with P2X7 ext.-FITC

Flow Cytometry:

0.5-10ml antibody / 1x106cells.

References:

1. Ding, Y. et al. (2000) J. Auton.Nerv.Syst. 81, 289.

2. Kim, M. et al. (2001) EMBO J. 20, 6347.

3. Chizh, B.A. et al. (2000) Pharmacol. Rev. 53, 553.

4. Nihei, O.K. et al. (2000) Mem.Inst.Oswaldo Cruz. 95, 415.