- 貨號 Cat. No.
- APR-004-AO
- 規格 Size
- 50 ul
Description
Anti-P2X7-ATTO-550
Epitope: |
Peptide (C)KIRK EFPKT QGQYS GFKYP Y, corresponding to amino acid residues 576-595 of rat P2X7 (Accession Q64663).
|
Putative epitope location: |
Intracellular, C-terminus. |
Homology: |
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.
Label: |
ATTO-550. Maximun absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540-565 nm range. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. |
|
Reactivity Confirmed: |
Rat, mouse. |
Applications:
 |
Western blotting of rat hippocampus lysate:
1. Anti-P2X7 antibody (#APR-004) (1:200).
2. Anti-P2X7 antibody, preincubated with the control peptide antigen.
|
Immunohistochemistry: |
Expression of P2X7 in rat pancreas. |
 |
Immunohistochemical staining of P2X7 in rat paraffin embedded endocrine and exocrine pancreas sections using Anti-P2X7-ATTO-550 antibody (#APR-004-AO) (1:20) (red). Staining is highly specific for endocrine cells of the Isle of Langerhans. Counterstain is Hoechst 33342 (blue).
|
1. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. |
2. Kim, M. et al. (2001) EMBO J. 20, 6347. |
3. Chizh, B.A. and Illes, P. (2001) Pharmacol. Rev. 53, 553. |
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP.
To date, this family is composed of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.
The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells, and microglia.
The P2X7 receptor mediates the release of proinflamatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3
The Anti-P2X7 antibody (#APR-004) can be used in Western blot analysis and immunohistochemical applications and recognizes P2X7 from rat, mouse, and human samples.
Alomone Labs is pleased to offer a new version of this antibody, Anti-P2X7-ATTO-550 antibody (#APR-004-AO), that is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. The Anti-P2X7-ATTO-550 antibody has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.
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