Anti-P2X4
- 貨號 Cat. No.
- APR-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-P2X4
| Product#: | APR-002 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)KKYK YVEDY EQGLS GEMNQ corresponding to amino acid residues 370-388 of rat P2X4 (Accession P51577). |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
Mouse - identical; human - 15/19 amino acid residues identical; chicken - 11/12 amino acid residues identical; Xenopus - 11/11 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-P2X4 antibody (#APR-002), (1:200). 2. Anti-P2X4 antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of P2X4 in rat brain |
 |
Immunohistochemical staining of P2X4 in rat brain red nucleus using Anti-P2X4 antibody (#APR-002). A, P2X4 (green) appears in fibers surrounding cell shapes (arrows). B, Calbindin 28K (red) appears in large neurons. C, merge of P2X4 and Calbindin 28K suggest variable density of P2X4 expressing fibers on red nucleus neurons. DAPI is used as the counterstain (blue). |
| References: |
| 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. |
| 2. Florenzano, F. et al. (2002) Neuroscience. 115, 425. |
| 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. |
| 4. Khakh, B.S. et al. (2001) Pharmacol Rev. 53, 107. |
| 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. |
| 6. Le, K.T. et al. (1998) J. Neurosci. 18, 7152. |
| 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. |
| 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. |
| 9. Kim, M. et al. (2001) EMBO J. 20, 6347. |
| 10. Inoue, K.et al. (2004) J. Pharmacol. Sci.94,112. |
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1î ‰2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini, two membrane-spanning domains and a large extracellular loop.
All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9
The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4
The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.
Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10
Alomone Labs offers a highly specific antibody directed against an intracellular epitope at the C-terminus of the rat P2X4 Receptor channel. TheAnti-P2X4 antibody (#APR-002) can be used in Western blot analyses and recognizes the P2X4 Receptor from rat samples.