Anti-P2X2
- 貨號 Cat. No.
- APR-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-P2X2
| Product#: | APR-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)SQQD STSTD PKGLA QL, corresponding to amino acid residues 457-472 of rat P2X2 (Accession P49653). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: | |
|
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates: 1, 2. Anti-P2X2 antibody (#APR-003) (1:200). 3, 4. Anti-P2X2 antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of P2X2 receptor in rat neocortex |
 |
Immunohistochemical staining of P2X2 receptor in rat neocortex using Anti-P2X2 antibody (#APR-003). P2X2 receptor expression (green fluorescence) appeared in axonal processes (arrows) that ascend toward the upper cortical layers (asterik). DAPI is used as the counterstain (blue). Expression of P2X2 receptor in rat hippocampus |
 |
Immunohistochemical staining of P2X2 receptor in rat hippocampus using Anti-P2X2 antibody (#APR-003). P2X2 receptor expression (green fluorescence) appeared in the apical dendrites (arrows) that extend into the striatum radiatum (asterik). DAPI is used as the counterstain (blue). |
| References: |
| 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. |
| 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. |
| 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. |
| 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. |
| 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. |
| 6. L? K.T. et al. (1998) J. Neurosci. 18, 7152. |
| 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. |
| 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. |
| 9. Kim, M. et al. (2001) EMBO J. 20, 6347. |
| 10. Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev. 53, 553 |
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate of their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1î ‰2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.
mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10
Alomone Labs offers a highly specific antibody directed against an intracellular epitope at the C-terminus of the rat P2X2 Receptor. The Anti-P2X2 antibody (#APR-003) can be used in Western blot analyses and recognizes the P2X2 Receptor from rat samples.