Anti-P2X1
- 貨號 Cat. No.
- APR-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-P2X1
| Product#: | APR-001 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (CS)DPV ATSST LGLQE NMRTS, corresponding to amino acid residues 382-399 of rat P2X1 (Accession P47824). |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, human. |
Applications:
| Western Blotting: |
 | Western blot analysis of human platelets lysate: 1. Anti-P2X1 antibody (#APR-001) (1:200). 2. Anti-P2X1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Rat heart paraffin section. |
| References: |
| 1. Prasad, M. et al. (2001) J. Physiol. 537, 667. |
| 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. |
| 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. |
| 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. |
| 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. |
| 6. L? K.T. et al. (1998) J. Neurosci. 18, 7152. |
| 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. |
| 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. |
| 9. Kim, M. et al. (2001) EMBO J. 20, 6347. |
| 10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182. |
| 11. Oury, C. et al. (2003) Blood 101, 3969. |
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1î ‰2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop.
All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis.3,4,10,11
Alomone Labs offers a highly specific antibody directed against an epitope at the intracellular C-terminus of the P2X1 receptor channel. The Anti-P2X1 antibody(#APR-001)can be used in Western blot analysis and immunohistochemical applications and recognizes P2X1 receptor from rat, mouse, and human samples.