- 貨號 Cat. No.
- ACC-061
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Orai2
Epitope: |
Peptide CPEPG HKGMD YRDWV RR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10). |
Putative epitope location: |
N-terminus, intracellular. |
Homology: |
Human and rat ?identical. |
Reactivity Confirmed: |
Human.
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Applications:
 |
Western blot analysis of lysates from HL-60 (human promyelocytic leukemia) (lanes 1 and 3) and Jurkat (human acute T cell leukemia) (lanes 2 and 4) cell lines:
1, 2. Anti-Orai2 antibody (#ACC-061), (1:200).
3, 4. Anti-Orai2 antibody preincubated with the control peptide antigen.
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Immunohistochemistry: |
Expression of Orai 2 in rat lung. |
 |
Immunohistochemical staining of a paraffin-embedded rat lung section using Anti-Orai2 antibody (#ACC-061), (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue arrow) and alveoli walls (red arrow). Hematoxylin was used as the counterstain.
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1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. |
2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. |
3. Feske, S. et al. (2006) Nature 441, 179. |
4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794. |
5. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548. |
6. Mercer, J.C. et al. (2006) J. Biol. Chem. 281, 24979. |
7. Gwack, Y. et al. (2007) J. Biol. Chem.282, 16232. |
Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest.
Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5
Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM16
Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7
Alomone Labs is pleased to offer a new antibody directed against an intracellular epitope of the mouse Orai2 channel. The Anti-Orai2 antibody (#ACC-061) can be used in Western blot and immunohistochemical applications and should recognize Orai2 from human, rat, and mouse samples.
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