Anti-NOX2 Antibody

Anti-NOX2 Antibody LS-B4010

NOX2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4010 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4010 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

NOX2 Antibody Details

Target: Human NOX2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

NOX2 Antibody Specificity

Immunogen: NOX2 antibody was raised against synthetic peptide from C-terminus of human CYBB (NP_000388.2, P04839). Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit (100%); Dog (92%).

Epitope: C-Terminus

Specificity: Human CYBB / gp91phox

NOX2 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.