Anti-NOTCH2 Antibody

Anti-NOTCH2 Antibody LS-B399

NOTCH2 Rabbit anti-Human Polyclonal (aa2396-2409) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B399 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B399 was determined to be 1:500. Learn more

NOTCH2 Antibody Details

Target: Human NOTCH2

Host: Rabbit

Reactivity: Human

Clonality: Polyclonal 

Purification: Sterile Filtration

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (1:500)

Western blot (WB) (1:400 - 1:2000)

Immunofluorescence (IF)

ELISA (1:30000 - 1:90000)

Optimal dilution to be determined by the researcher.

 

NOTCH2 Antibody Specificity

Immunogen: NOTCH2 antibody was raised against synthetic peptide from human NOTCH2.

Epitope: aa2396-2409

Specificity: Amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.

NOTCH2 Antibody Packaging

Presentation: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For research use only.