Anti-NOTCH1 Antibody

Anti-NOTCH1 Antibody LS-B398

NOTCH1 Rabbit anti-Human Polyclonal (aa2488-2502) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B398 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B398 was determined to be 1:500. Learn more

NOTCH1 Antibody Details

Target: Human NOTCH1

Host: Rabbit

Reactivity: Human

Clonality: Polyclonal 

Purification: Antiserum

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (1:500)

Western blot (WB) (1:2000 - 1:10000)

ELISA (1:20000 - 1:100000)

Optimal dilution to be determined by the researcher.

 

NOTCH1 Antibody Specificity

Immunogen: NOTCH1 antibody was raised against synthetic peptide from human NOTCH1.

Epitope: aa2488-2502

Specificity: Amino acid residues 2488-2502 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.

NOTCH1 Antibody Packaging

Presentation: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions: For research use only.