Anti-Nogo Receptor
- 貨號 Cat. No.
- ANT-008
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nogo Receptor
| Product#: | ANT-008 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide(C)GDSPPGNGSGPRHIND corresponding to amino acid residues 366-381 of the human Nogo receptor (Accession Q9BZR6). |
 |
| Putative epitope location: |
| Extracellular. |
| Homology: |
Mouse, rat- 15 out of 16 residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysate:
|
| Immunohistochemistry: |
 |
Immunohistochemical staining of Nogo receptor with Anti-Nogo receptor antibody (#ANT-008) in rat cerebellum. (A) Nogo receptor (red) appears in Purkinje cells (arrows). (B) Staining of Purkinje nerve cells with mouse anti calbindin D28K (a calcium binding protein, green). (C) Confocal merge of Nogo receptor and calbindin D28K demonstrates some co-localization of these proteins. |
| Immunocytochemistry: |
 |
(A) Live cultured rat cerebellar granule cells were stained with Anti-Nogo Receptor antibody(#ANT-008)followed by goat-anti-rabbit Alexa-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue). (B) Live view of the same field as in (A). |
| References: |
| 1. Fournier, A.E. et al. (2001) Nature 409, 341. |
| 2. Prinjha, R. et al. (2000) Nature 403, 383. |
| 3. GrandPr? T. et al. (2000) Nature 403, 439. |
| 4. Domeniconi, M. et al. (2002) Neuron 35, 283. |
| 5. Wang, K.C. et al. (2002) Nature 417, 941. |
| 6. Wang, K.C. et al. (2002) Nature 420, 74. |
| 7. Mi, S. et al. (2004) Nat. Neurosci. 7, 221. |
The Nogo receptor is a leucine-rich repeat (LRR) containing protein with a glycosylphosphatidylinositol (GPI) anchored C-terminus. The receptor was identified on the basis of its ability to bind with high affinity to Nogo-A a member of the reticulon family expressed in the cell membrane of oligodendrocytes.1
Nogo-A attracted attention when it was demonstrated that it is a myelin protein capable of inhibiting axonal growth following nerve injury.2,3 Other myelin proteins also identified as axonal growth inhibitors are the myelin-associated glycoprotein (MAG) a sialic-dependent immunoglobulin-like family member lectin (SIGLEC) and the oligodendrocyte myelin glycoprotein (OmgP) which is a GPI-anchored membrane protein. Remarkably, despite their structural diversity, all three axonal growth inhibitors bind to the Nogo receptor with high affinity.4,5
Since the Nogo receptor is a GPI-anchored protein it was expected that it would require another protein component to transduce the Nogo-A binding information into the responding neurons interior. Indeed, it was shown that the neurotrophin receptor p75 (p75NTR) interacts with Nogo receptor and a third protein termed LINGO-1 to mediate axonal growth inhibition signaling.6,7
We are pleased to offer a new antibody directed against a highly conserved epitope situated in the linker region between the LRRs and the GPI anchor. The antibody will work with human, mouse and rat samples and has been tested in Western blot, immunocytochemistry and immunohistochemistry techniques.