Anti-NMDA Receptor 2B (NR2B) (extracellular)
- 貨號 Cat. No.
- AGC-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-NMDA Receptor 2B (NR2B) (extracellular)
| Product#: | AGC-003 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acids 323-337 of rat NMDAR2B (Accession Q00960). |
 |
| Putative epitope location: |
| Extracellular, N-terminus. |
| Homology: |
| Human, mouse- identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain lysate:
|
| Immunohistochemistry: |
 |
Immunohistochemical staining of NMDAR2B in rat hippocampal CA1region. Frozen rat brain sections were stained with Anti-NMDAR2B antibody (#AGC-003) (1:100). (A) NMDAR2B (green) appears in the pyramidal layer of CA1. (B) Staining of Neurofilament 200 (NF, red) identifies neuronal processes. (C) Confocal merge demonstrates localization of NMDAR2B in cells; not in processes. |
 |
Immunohistochemical staining of NMDAR2B in rat parietal cortex. Frozen rat brain sections were stained with Anti-NMDAR2B antibody (#AGC-003) (1:100). (A) NMDAR2B (green) appears in the pyramidal layer of layer 5. (B) Staining of Neurofilament 200 (NF, red) identifies neuronal processes. (C) Confocal merge demonstrates localization of NMDAR2B in cells. |
| Immunoprecipitation: |
 | Immunoprecipitation of Rat brain lysate with Anti-NMDAR2B:
Red arrow indicates the NMDAR2B protein while the black arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-NMDAR2B. |
| References: |
| 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. |
| 2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161. |
| 3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673. |
| 4. Mayer, M.L. (2006) Nature 440, 456. |
The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.
The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.
The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.
Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinsonî–¸ and Alzheimerî–¸ disease.
NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.
Alomone Labs has produced a new antibody against a conserved extracellular epitope of the rat NMDAR2B subunit. The antibody has been tested in Western blot and immunohistochemistry applications.