Anti-NMDA Receptor 2A (NR2A) (extracellular)
- 貨號 Cat. No.
- AGC-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-NMDA Receptor 2A (NR2A) (extracellular)
| Product#: | AGC-002 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide GHSHDVTERELRN(C), corresponding to amino acids 41-53 of rat NMDAR2A (Accession Q00959). |
| Putative epitope location: |
| Extracellular, N-terminus. |
 |
| Homology: |
Mouse- identical; Human- 12/13 residues identical. |
| Reactivity Confirmed: |
| Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain lysate:
|
| Immunohistochemistry: |
 |
Immunohistochemical staining of NMDAR2A with Anti-NMDAR2A antibody (#AGC-002) in rat hippocampal dentate gyrus. A. NR2A (green) appears diffusely in the outer molecular layer of dentate gyrus (Out Mol.) and in cells along the subgranular layer (arrows). B. Staining of parvalbumin (PV, red) identifies interneurons in the dentate gyrus. C. Confocal merge demonstrates localization of PV in some neurons with NR2A expression in the dentate gyrus. |
| Immunocytochemistry: |
 |
| Immunocytochemical staining of live intact rat C6 glioma cells with Anti-NMDAR2A antibody. C6 glioma cells were stained with Anti-NMDAR2A antibody (#AGC-002)(1:100) followed by goat-anti-rabbit Alexa-555 secondary antibody (red staining). Cell nuclei were stained with the cell permeable dye Hoechst 33342 (blue staining). |
| Immunoprecipitation: |
 | Immunoprecipitation of rat brain lysate with Anti-NMDAR2A:
Black arrow indicates the NMDAR2A protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDAR2A antibody. |
| References: |
| 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. |
| 2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161. |
| 3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673. |
| 4. Mayer, M.L. (2006) Nature 440, 456. |
The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.
The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.
The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.
Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinsonî–¸ and Alzheimerî–¸ disease.
NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.
Alomone Labs has produced a new antibody against a conserved extracellular epitope of the rat NMDAR2A subunit. The antibody also recognizes mouse and human samples and has been tested in Western blot and immunohistochemistry applications.