Anti-NMDA Receptor 1 (NR1) (extracellular)

 

Product#:

AGC-001

Sizes:

50 ml

0.2 ml

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.



The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.



The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg²⁺. Neuronal depolarization relieves the Mg²⁺ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca²⁺ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.



Ca²⁺ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinsonî–¸ and Alzheimerî–¸ disease.



NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.



Alomone Labs has produced a new antibody against a conserved extracellular epitope of the rat NMDAR1 subunit. The antibody also recognizes mouse and human samples.

Host:

Rabbit

Epitope:

Peptide ETEKPRGYQMSTRLK(C), corresponding to amino acids 385-399 of rat NMDAR1 (Accession P35439).

Putative epitope location:

Extracellular, N-terminus.

Homology:

Human, mouse- identical.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Western blotting of rat brain lysate: Anti-NMDAR1 antibody (#AGC-001) (1:600). Anti-NMDAR1 antibody, preincubated with the control peptide antigen.

References:

1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.

2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161.

3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673.

4. Mayer, M.L. (2006) Nature 440, 456.