Anti-NHERF-1
- 貨號 Cat. No.
- APZ-006
- 規格 Size
- 50 μl 0.2ml
Description
Anti-NHERF-1
(Na+/H+ exchanger regulatory factor 1, EBP-50)
| Product#: | APZ-006 |
| Sizes: |
| 50 ml |
| 0.2 ml |
The Na+/H+ exchanger regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic
protein adaptor that recruits a wide variety of cellular proteins.
Many of the interacting proteins do so through the two tandem PDZ domains
(protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1)
and the C-terminal ERM (ezrin, radixin, moesin) binding region.
NHERF-1 was first identified as an adaptor necessary for the function of the Na+/H+
exchanger isoform 3(NHE3) in renal apical cells.1 Since then it has been identified
in cells of epithelial origin in several tissues such as gastrointestinal and lung.
NHERF-1 has been shown to interact with a growing number of proteins including ion
channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR),
phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases
(YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.2
Recently it has been showed that NHERF-1 expression was elevated in breast tumors compared
to the expression in adjacent normal tissue.3
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19). |
| Putative epitope location: |
| Between the PDZ2 and the ERM binding domains. |
 |
| Homology: |
Mouse 13/14 residues identical; human 12/14 residues identical. Specificity: |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
| Rat kidney membranes (1:200-1:500). |
 | Western blotting of rat kidney membranes: 1. Anti-NHERF-1 antibody (1:200). 2. Anti NHERF-1 antibody, preincubated with the control peptide |
Control antigen included in price
| References: |
1. | Reczek, D. et al. (1997) J. Cell Biol. 139, 169. |
2. | Voltz, J.W. et al. (2001) Oncogene 20, 6309. |
3. | Stemmer-Rachamimov, A.O. et al. (2001) Am. J. Pathol. 158, 57. |