Anti-NFX1 Antibody

Anti-NFX1 Antibody LS-B3576

NFX1 Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3576 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3576 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. Learn more

NFX1 Antibody Details

Target: Human NFX1

Host: Rabbit

Reactivity: Human

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (0.2 - 1)

Optimal dilution to be determined by the researcher.

 

NFX1 Antibody Specificity

Immunogen: NFX1 antibody was raised against synthetic peptide from internal region of human NFX1 (NP_002495.2, Q12986). Percent identity by BLAST analysis: Human (100%); Rat, Dog, Bovine, Rabbit (85%).

Epitope: Internal

Specificity: Human NFX1

NFX1 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.