Anti-NFIC Antibody

Anti-NFIC Antibody LS-B4029

NFIC Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4029 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4029 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. Learn more

NFIC Antibody Details

Target: Human NFIC

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

NFIC Antibody Specificity

Immunogen: NFIC antibody was raised against synthetic peptide from internal region of human NFIC (NP_995315.1, Q6FI30). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%); Chicken (93%); Xenopus (86%).

Epitope: Internal

Specificity: Human NFIC

NFIC Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: +4°C short term (up to one week); -20°C long term (regardless of reconstitution); aliquot to avoid freeze/thaw cycles after reconstitution

Restrictions: For research use only.