Anti-NFAT2 antibody
- 貨號 Cat. No.
- ab25916
- 規格 Size
- 100 µg
- 品牌 Brand
- Abcam
Description
pnbsp;Product nameAnti-NFAT2 antibody/p
divSee all NFAT2 primary antibodiesDescriptionRabbit polyclonal to NFAT2SpecificityThe amino acid sequence used is 100% identical in human NFATc1A, B, C and E isoforms. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon the cell lines.Tested applicationsIHC-P, WBmore details Species reactivityReacts with: Mouse, Rat, Chicken, Human, Chimpanzee/div
divImmunogen/div
divSynthetic peptide:/div
divNKRKYSLNGRQPPYSPHHS/div
div, corresponding to amino acids 263-282 of Human NFATc1/div
divRun BLAST with nbsp;BLAST the sequence with ExPASy Run BLAST with nbsp;BLAST the sequence with NCBI/div
divPositive control/div
divRamos cell lysate/div
divProperties/div
divFormLiquidStorage instructionsStore at +4deg;C short term (1-2 weeks). Aliquot and store at -20deg;C long term. Avoid repeated freeze / thaw cycles.Storage bufferPreservative: 0.05% Sodium Azide/div
divConstituents: 0.05% BSA, PBS/div
divConcentration100 micro;g at 0.5 mg/mlnbsp;/div
divClonality Polyclonal IsotypeIgG Research Areas Immunology Adaptive Immunity T Cells Non-CD Signal Transduction Signaling Pathway Nuclear Signaling NFATS Epigenetics and Nuclear Signaling Transcription Other factors Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFATs Cardiovascular Heart Cardiogenesis Transcription factors/regulators/div
divApplications/div
divOur Abpromise guarantee covers the use of ab25916 in the following tested applications./div
divThe application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user./div
divApplicationspan class=Apple-tab-span style=white-space:pre /spanAbreviewsspan class=Apple-tab-span style=white-space:pre /spanNotes/div
divIHC-Pspan class=Apple-tab-span style=white-space:pre /spanUse a concentration of 5 micro;g/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol./div
divWBspan class=Apple-tab-span style=white-space:pre /spanUse a concentration of 1 - 3 micro;g/ml. Predicted molecular weight varies with isoform./div
divTarget/div
divFunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.Tissue specificityExpressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.Sequence similaritiesContains 1 RHD (Rel-like) domain.DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors./div
divThe N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus./div
divIsoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.Post-translational/div
divmodificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin.Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription./div
divInformation by UniProt/div
divDatabase links/div
divEntrez Gene: 4772 Human/div
divEntrez Gene: 18018 Mouse/div
divEntrez Gene: 100361818 Rat/div
divOmim: 600489 Human/div
divSwissProt: O95644 Human/div
divSwissProt: O88942 Mouse/div
divUnigene: 534074 Human/div
divUnigene: 701518 Human/div
divsee allAlternative names/div
divcytoplasmic 1 antibody/div
divMGC138448 antibody/div
divNAFATC antibody/div
divsee all/div
divAnti-NFAT2 antibody images/div
divWestern blot - NFAT2 antibody (ab25916)/div
divWestern blot - NFAT2 antibody (ab25916)/div
divWestern blot analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NFAT2 antibody(ab25916)/div
divImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NFAT2 antibody(ab25916)/div
divIHC image of ab25916 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25916, 5micro;g/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX./div
divnbsp;/div
divFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times./div
divnbsp;/div
divSee all NFAT2 primary antibodiesDescriptionRabbit polyclonal to NFAT2SpecificityThe amino acid sequence used is 100% identical in human NFATc1A, B, C and E isoforms. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon the cell lines.Tested applicationsIHC-P, WBmore details Species reactivityReacts with: Mouse, Rat, Chicken, Human, Chimpanzee/div
divImmunogen/div
divSynthetic peptide:/div
divNKRKYSLNGRQPPYSPHHS/div
div, corresponding to amino acids 263-282 of Human NFATc1/div
divRun BLAST with nbsp;BLAST the sequence with ExPASy Run BLAST with nbsp;BLAST the sequence with NCBI/div
divPositive control/div
divRamos cell lysate/div
divProperties/div
divFormLiquidStorage instructionsStore at +4deg;C short term (1-2 weeks). Aliquot and store at -20deg;C long term. Avoid repeated freeze / thaw cycles.Storage bufferPreservative: 0.05% Sodium Azide/div
divConstituents: 0.05% BSA, PBS/div
divConcentration100 micro;g at 0.5 mg/mlnbsp;/div
divClonality Polyclonal IsotypeIgG Research Areas Immunology Adaptive Immunity T Cells Non-CD Signal Transduction Signaling Pathway Nuclear Signaling NFATS Epigenetics and Nuclear Signaling Transcription Other factors Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFATs Cardiovascular Heart Cardiogenesis Transcription factors/regulators/div
divApplications/div
divOur Abpromise guarantee covers the use of ab25916 in the following tested applications./div
divThe application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user./div
divApplicationspan class=Apple-tab-span style=white-space:pre /spanAbreviewsspan class=Apple-tab-span style=white-space:pre /spanNotes/div
divIHC-Pspan class=Apple-tab-span style=white-space:pre /spanUse a concentration of 5 micro;g/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol./div
divWBspan class=Apple-tab-span style=white-space:pre /spanUse a concentration of 1 - 3 micro;g/ml. Predicted molecular weight varies with isoform./div
divTarget/div
divFunctionPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.Tissue specificityExpressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.Sequence similaritiesContains 1 RHD (Rel-like) domain.DomainRel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors./div
divThe N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus./div
divIsoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.Post-translational/div
divmodificationsPhosphorylated by NFATC-kinase; dephosphorylated by calcineurin.Cellular localizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription./div
divInformation by UniProt/div
divDatabase links/div
divEntrez Gene: 4772 Human/div
divEntrez Gene: 18018 Mouse/div
divEntrez Gene: 100361818 Rat/div
divOmim: 600489 Human/div
divSwissProt: O95644 Human/div
divSwissProt: O88942 Mouse/div
divUnigene: 534074 Human/div
divUnigene: 701518 Human/div
divsee allAlternative names/div
divcytoplasmic 1 antibody/div
divMGC138448 antibody/div
divNAFATC antibody/div
divsee all/div
divAnti-NFAT2 antibody images/div
divWestern blot - NFAT2 antibody (ab25916)/div
divWestern blot - NFAT2 antibody (ab25916)/div
divWestern blot analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NFAT2 antibody(ab25916)/div
divImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-NFAT2 antibody(ab25916)/div
divIHC image of ab25916 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25916, 5micro;g/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX./div
divnbsp;/div
divFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times./div
divnbsp;/div
