Anti-Neurotrophin-4

Anti-Neurotrophin-4

Product#:

ANT-004

Sizes:

50 μl

0.2 ml

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble,  

basic growth factors which regulate neuronal development, maintenance, survival and death in  

the CNS and the PNS.¹ Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical,  

stellate and celiac ganglion,² T-cells³ and is synthesized by keratinocytes.⁴



The structural hallmark of all the neurotrophins is the characteristic arrangement of the  

disulfide bridges known as the cysteine knot, which has been found in other growth factors  

such as PDGF.⁵ The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to  

promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,⁶  

to promote growth and remodeling of adult motor neuron innervation,⁷ to be anterograde  

survival factors for postsynaptic cells⁸ and to protect against apoptotic neuronal death.⁹  

The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for  

NT-4 and BDNF, and p75 which binds all the neurotrophins.¹⁰

Host:

Rabbit.

Description:

Polyclonal antibody raised in rabbit, affinity purified on immobilized antigen.

Epitope:

peptide LGEVPAAGGS PL (C).  (Accession number P34130 ).

Putative epitope location:

Amino acid residues corresponding to residues 43-52 of mature human NT-4.   (123-132 of the precursor NT-4).

Homology:

Rat - identical.

Applications:

Immunohistochemistry:

Immunohistochemical staining of NT4/5 using Anti-NT4/5 in mouse spinal cord.   The spinal ventral horn (VH) was very lightly stained. However, in the perimeter  of the white matter (WM) there were NT4/5 immunoreactive cells with a small soma   (triangles) and some had a stained process (arrows).

Western Blotting:

Western blotting of human recombinant NT-4 (10 ng).

Anti-NT-4, 1:200. Anti-NT-4, preincubated with the control peptide antigen .

Control antigen included in price

References:

1.	Roux, P. et al. (2002) Prog. Neurobiol. 67, 203.
2.	Roosen, A. et al. (2001) J. Neurosci. 21, 3073.
3.	Moalem, G. et al. (2002) J. Autoimmun. 15, 331.
4.	Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515.
5.	McDonald, N.Q. et al. (1991) Nature 354, 411.
6.	DeFazio, R.A. et al. (2000) Neuroscience, 99, 297.
7.	Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56.
8.	Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485.
9.	Lobner, D. and Ali, C. (2002) Brain Res. 954, 42.
10.	Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.