Anti-Neurotrophin-3
- 貨號 Cat. No.
- ANT-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Neurotrophin-3
| Product#: | ANT-003 |
| Sizes: |
| 50 μl |
| 0.2 ml |
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble,
basic growth factors which regulate neuronal development, maintenance, survival and death in
the CNS and the PNS.1 The structural hallmark of all the neurotrophins is the characteristic
arrangement of the disulfide bridges known as the cysteine knot, which has been found in other
growth factors such as PDGF.2 The rat and human forms of Neurotrophin-3 (NT-3) are 96% homologous.
NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3
to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical
role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in
isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated
by two receptors: TrkC, which is specific for NT-3, and p75, which binds all the neurotrophins.7
| Host: |
| Rabbit. |
| Description: |
| Polyclonal antibody raised in rabbit, affinity purified on immobilized antigen. |
| Epitope: |
| Peptide GEIKTGNSPV(C). |
| Putative epitope location: |
| Amino acid residues corresponding to residues 39-48 of mature human NT-3. (residues 177-186 of the NT-3 precursor). |
 |
Applications:
| Immunohistochemistry: |
| Immunohistochemical staining of NT-3 using Anti-NT-3 (#ANT-003) in mouse spinal chord. The spinal ventral horn was stained diffusely with some extensions (arrows) into the white matter (WM). In the WM there were NT-3 immunoreactive cells that were probably glia (triangles). |
 |
| Western Blotting: |
| Western blotting using anti-NT-3 antibody (1:1000) (1-4) or anti-NT-3 antibody, preincubated with the control antigen peptide (5). |
 |
|
Control antigen included in price
| References: |
1. | Roux, P. et al. (2002) Prog. Neurobiol. 67, 203. |
2. | McDonald N.Q. et al. (1991) Nature 354, 411. |
3. | Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. |
4. | Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. |
5. | Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221. |
6. | Kimitsuki, T. et al. (2003) Auris. Nasus Larynx. 30, 141. |
7. | Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. |