Anti-Neurotensin Receptor 2
- 貨號 Cat. No.
- ANT-016
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Neurotensin Receptor 2
| Product#: | ANT-016 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| PeptideCGEQHSLVPLPQEAPE corresponding to residues 377-392 of rat Neurotensin Receptor 2 (Accession Q63384 ). |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
Mouse -14/16 residues identical. Unlikely to recognize human samples. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysates: 1. Anti-Neurotensin Receptor 2 antibody (#ANT-016), 1:200. 2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen. |
 | Western blot analysis of rat dorsal root ganglion lysates: 1. Anti-Neurotensin Receptor 2 antibody (#ANT-016), 1:200. 2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen. |
 | Western blot analysis of mouse brain lysates: 1. Anti-Neurotensin Receptor 2 antibody (#ANT-016), 1:400. 2. Anti-Neurotensin Receptor 2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Immunohistochemical staining of Neurotensin Receptor 2 in rat mediobasal (MB) hypothalamus. A. Perfusion-fixed brain frozen sections were stained with Anti-Neurotensin Receptor 2 (#ANT-016) antibodies (1:100) (green staining). Neurotensin Receptor 2 (NTS2) appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTS2 is not localized in astrocytes. Blue is DAPI counterstain. |
 |
Expression of Neurotensin Receptor 2 (NTR2) in rat stomach. Paraffin-embedded rat stomach sections were stained with Anti-Neurotensin Receptor 2 (#ANT-016) antibodies (1:50) (brown staining). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxylin is used as the counterstain. |
| Immunocytochemistry: |
 |
Immunocytochemical staining of Neurotensin Receptor 2 in rat C6 glioma cells: A. Paraformaldehyde fixed and permeabilized rat C6 glioma cells were stained with Anti-Neurotensin Receptor 2 antibody (#ANT-016) (1:200) followed by Alexa-555 conjugated goat-anti-rabbit- secondary antibody (orange staining). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as in (A). |
Neurotensin receptor 2 (NTS2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.
NTS2 and NTS1 belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily while the third neurotensin receptor NTS3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTS1 and NTS3 bind neurotensin with high affinity while NTS2 binds it with low affinity.1
NTS2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization. However, the exact signaling mechanisms subsequent to NTS2 activation appear to depend on the species and on the cellular system in which the receptor is studied.
NTS2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acidsecretion.2
The most established physiological role of NTS2 is its role in pain transmission as NTS2-defficient mice showed that NTS2 is the likely mediator of the neurotensin-induced analgesic responses.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat NTS2. Anti-Neurotensin Receptor-2 antibody (#ANT-016) can be used in Western blot, immunohistochemical and immunocytochemical applications, and recognizes NTS2 receptor from rat and mouse samples.