Anti-Neuropeptide Y5 Receptor
- 貨號 Cat. No.
- ANR-025
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Neuropeptide Y5 Receptor
| Product#: | ANR-025 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide SFIRKHRRRYSKKTAC corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634 ).
|
 |
| Putative epitope location: |
3rd intracellular loop. |
| Homology: |
| Mouse, bovine and Canis ?identical. Human ?15/16 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membrane: 1. Anti-NPY5R antibody (#ANR-025), (1:200). 2. Anti- NPY5R antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Neuropeptide Y5 Receptor in rat striatum |
 |
Immunohistochemical staining of Neuropeptide Y5 Receptor in rat striatum using Anti-NPY5R antibody (#ANR-025). NPY5R (red) appears in the striatal matrix (white asterik) and in nerve cells in the matrix (vertical arrows). B, Parvalbumin (green) appears in the striatal matrix. C, merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).
|
| Immunocytochemistry: |
Expression of NPY5R in Rat Dorsal Root Ganglion primary culture |
 |
Immunocytochemical staining of NPY5R in a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R antibody (#ANR-025), (1:100) followed by Alexa-488-conjugated goat anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
Magnification: A-C: x20 E-F: x100 |
Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2
NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.
Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2
NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Neuropeptide Y5 receptor (NPY5R). The Anti-NPY5R antibody (#ANR-025) can be used for Western blot analysis, immunohistochemical and immunocytochemical staining applications. It has been designed to recognize NPY5R from rat samples, and based on the sequence homology is expected to recognize NPY5R from human and mouse samples as well.