Anti-Nestin Antibody

Anti-Nestin Antibody LS-B51

Nestin Mouse anti-Human Monoclonal (aa1464-1614) (10C2) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B51 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B51 was determined to be 2.5 ug/ml. Learn more

Nestin Antibody Details

Target: Human Nestin

Host: Mouse

Reactivity: Human

Non-Reactivity: Mouse, Rabbit, Rat

Clonality: IgG1,k Monoclonal [10C2] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (2.5)

Western blot (WB) (1:1000 - 1:5000)

Immunofluorescence (IF)

Optimal dilution to be determined by the researcher.

 

Nestin Antibody Specificity

Immunogen: Nestin antibody was raised against a fragment of the human nestin protein corresponding to residues 1464-1614

Epitope: aa1464-1614

Specificity: Human nestin.

Nestin Antibody Packaging

Presentation: PBS, 0.1% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For research use only.