Anti-Navb2
- 貨號 Cat. No.
- ASC-007
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Navb2
| Product#: | ASC-007 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)KTEE EGKTD GEGNA EDGAK, corresponding to amino acid residues 197-215 of rat b2 subunit of voltage-gated Na+ channels (Accession P54900) |
 |
| Putative epitope location: |
| Intracellular, C-terminus. |
| Homology: |
Human, mouse - 17/19 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of Navb2 in rat brain membranes under reducing (1,2) or non-reducing (3,4) conditions.* 1,3. Anti-Navb2 antibody (#ASC-007), (1:200). 2,4. Anti-Navb2, preincubated with the control peptide antigen. * Navb2 is disulfide-linked to a subunit of voltage-gated Na+ channel (Schmidt, J.W., and Catterall, W.A. (1986) Cell 46, 437. |
| Immunohistochemistry: |
Expression of Navb2 in Mouse Cerebellum |
 |
Immunohistochemical staining of sodium beta2 (Navb2) channel in mouse cerebellum using Anti-Navb2 antibody (#ASC-007). A, Navb2channel (red) appears in Purkinje cells (vertical arrows) and in small cells in the molecular layer (horizontal arrows, Mol). B, staining of Purkinje nerve cells with mouse anti calcium binding protein (CBD28k, green) demonstrates the restriction of Navb2 to cell bodies. C, Confocal merge of Navb2 and CBD28k. Expression of Navb2 in Rat Cerebellum |
 |
Immunohistochemical staining of sodium beta2 (Navb2) channel in rat cerebellum using Anti-Navb2 antibody (#ASC-007). .A, Navb2 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol). B, staining of Purkinje nerve cells with mouse anti calcium binding protein (CBD28k, green) demonstrates the restriction of Nav b2 to cell bodies. C, Confocal merge of Navb2 and CBD28k |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 3. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 4. Catterall, W. A. (2000) Neuron 26, 13. |
| 5. Chen, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 17072. |
| 6. Schmidt, J. W., and W. A. Catterall. (1986) Cell 46, 437. |
| 7. Lopez-Santiago,L, F. (2006) The Journal of Neuroscience 6, 7984. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.2,3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b-subunits (Nav b-subunit).4
The Nav b-subunit gene family consists of four members: b1 (SCN1B), b2 (SCN2B), b3 (SCN3B), and b4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus.They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5
Nav b-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5
In the adult central nervous system and heart, sodium channels are associate with b1-b4 subunits, whereas in adult skeletal muscle they are associate only with the b1 subunit.4,5
This association appears to be a late event in sodium channel biosynthesis.6
Expression of b2-subunits increases in sensory neurons following nerve injury. b2 deficient mice develop less mechanical allodynia in compare to wild type mice, suggesting a role for b2 subunit in pain sensation. 7
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the Nav b2-subunit. The Anti-Nav b2 (#ASC-007) can be used for Western Blotting and Immunohistochemical applications. It has been designed to recognize Nav b2-subunit from human, rat and mouse samples.