Anti-Nav1.9
- 貨號 Cat. No.
- ASC-017
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.9
| Product#: | ASC-017 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide CNGDLSSLDVAKVKVHND corresponding to residues 1748-1765 of rat Nav1.9 (Accession O88457 ). |
 |
| Putative epitope location: |
C-Terminus, Intracellular. |
| Homology: |
| Mouse ?15/18 residues identical, human-14/18 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
| Western blotting of rat DRG lysates. |
 | Western blotting of rat DRG lysates: 1. Anti-Nav1.9 antibody, 1:200.
|
| Immunohistochemistry: |
| Rat embryo DRG formalin frozen sections. |
 |
| Staining of Nav1.9 in rat dorsal root ganglion (DRG) with Anti-Nav1.9 antibody (#ASC-017). Cells within the DRG were stained (see solid line frame enlarged in B) as well as fibers and the area of entry of dorsal root into spinal cord (see dashed line frame enlarged in C). The counterstain in B and C is DAPI, a fluorescent dye visualized in the UV range. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 3. Lai, J. et al (2003) Curr.Opin. Neurobiol 13, 291. |
| 4. Fang, X. et al (2002) J. Neurosci. 22, 7425. |
| 5. Fjell, J. et al (2000) NeuroReport 11, 199. |
| 6. Blum, R. et al (2002) Nature 419, 687. |
Voltage-gated Na+ channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-1.9. 2-3 The expression of the a subunits is developmentally and tissue specific.
The Nav channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5
TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant Nav channels are expressed in dorsal root ganglion (DRG) neurons, Nav1.8 and Nav1.9. The Nav1.9 channel (also called NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5
Recently, it has been shown that BDNF activated Nav1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone. 6