Anti-Nav1.7
- 貨號 Cat. No.
- ASC-008
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.7
| Product#: | ASC-008 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat Nav1.7 (Accession O08562). |
 |
| Putative epitope location: |
Intracellular loop between I and II domains. |
| Homology: |
| Rabbit-14/15 amino acid residues identical. Human and mouse - 13/15 amino acid residues identical. |
| Reactivity Confirmed: |
Rat. |
Applications:
| Western Blotting: |
 | Western blotting of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4): 1,2. Anti-Nav1.7 antibody (#ASC-008), (1:200). 3,4. Anti- Nav1.7 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Nav1.7 in rat Dorsal Root Ganglion |
 |
Immunohistochemical staining of Nav1.7 channel in rat dorsal root ganglion (DRG) using Anti-Nav1.7 antibody (#ASC-008 ). A, Nav1.7 channel (red) in DRG neurons. B, Staining with mouse anti Parvalbumin (green) in the same DRG section. C, Confocal merge of Nav1.7 and Parvalbumin demonstrates existence of the parvalbumin calcium binding protein in a subset of neurons expressing Nav1.7 (arrows). |
| Immunocytochemistry: |
 |
Immunocytochemical staining of Nav1.7 in rat dorsal root ganglion (DRG) primary culture. Paraformaldehyde-fixed and permeabilized DRG cells were stained using Anti-Nav1.7 antibody (#ASC-008), (1:200) followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. |
| 8. Catterall, W.A. et al. (2005) Pharmacol Rev. 57, 397. |
| 9. Yang, Y. et al. (2004) J. Med. Genet. 41, 171. |
| 10. Nassar, M. A. et al. (2004) Proc. Natal. Acad. Sci. U.S.A. 101, 12706. |
| 11. Cummins, T. R. et al (2004) J. Neurosci. 24, 8232. |
| 12. Dray, A. (2008) Br. J. Anaesth. 101, 48. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6-8
Nav1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the Nav1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss, or function mutations in Nav1.7 channels, leads to complete ablation of pain perception in humans.12 These recent findings highlight the role of this Nav isoform and the subset of DRG neurons that express this channel in physiological pain sensation.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.7 channel. The Anti-Nav1.7 antibody (#ASC-008) can be used for Western Blotting, Immunohistochemical and Immunocytochemical applications. It has been designed to recognize Nav1.7 from rat, human and mouse samples.