Anti-Nav1.6
- 貨號 Cat. No.
- ASC-009
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.6
| Product#: | ASC-009 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide CIANH TGVDI HRNGD FQKNG, corresponding to amino acid residues 1042-1061 of or rat Nav1.6 (Accession O88420). |
 |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse - identical, human - 19/20 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of Nav1.6 in rat brain membrane: 1. Anti-Nav1.6 antibody (#ASC-009), (1:200). 2. Anti-Nav1.6 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Nav1.6 in Mouse Hippocampus |
 |
Immunohistochemical staining of Nav1.6 channel in mouse hippocampus using Anti-Nav1.6 antibody (#ASC-009). NaV1.6 (green) was robustly expressed in the CA1 pyramidal layer (white arrows). Staining with mouse anti parvalbumin (red), a marker of interneurons, revealed that NaV1.6 appeared in some interneurons (white arrow in C) but was not restricted to interneurons. The blue is the DAPI is a general cell marker. |
| Immunocytochemistry: |
Expression of Nav1.6 in Rat Dorsal Root Ganglion Primary Culture |
 |
A. Paraformaldehyde-fixed and permeabilized DRG cells were stained using Anti-Nav1.6 antibody (#ASC-009), (1:200) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merge image of panels A and B. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. |
| 8. Catterall, W.A. et al. (2005) Pharmacol Rev. 57, 397. |
| 9. Boiko, T. et al. (2001)Neuron 30, 91. |
| 10. Caldwell et al. (2000) Proc. Natl. Acad. Sci. 97, 5616. |
| 11. Raman et al. (1997) J. Neurosci. 17, 4517. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6,7
Nav1.6 is highly expressed in adult brainand localized at high density in nodes of Ranvier and axon initial segments and at lower density in dendrites and cell bodies of some neurons. Nav1.6 channels are also expressed at high levels in cerebellar Purkinje neurons. 8-11
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.6 channel. The Anti-Nav1.6 antibody (#ASC-009) can be used for Western Blotting. It has been designed to recognize Nav1.6 from rat, human and mouse samples.