Anti-Nav1.5

 

Product#:

ASC-005

Sizes:

50 ml

0.2 ml

Voltage-gated Na⁺ channels (Nav) are responsible for myocardial conduction and  

maintenance of the cardiac rhythm and are essential for the generation of action  

potentials and cell excitability.¹  

Dysfunction or disregulation of cardiac sodium channels can cause several disorders,  

including cardiac arrhythmias.  

The majority of Na⁺ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive Nav1.5.² 

The putative structure of Na_V1.5 consists of four homologous domain (I-IV),  

each containing six transmembrane segments (S1-S6). 

Mutations in the C-terminus of Na_V1.5 were described in connection to Long QT  

syndrome and Brugada syndrome.^1-2 

Recent data have demonstrated selective expression of Na_V1.5 in the mouse central 

nervous system and implicated a role for Na_V1.5 in the physiology of the central 

nervous system.¹

Host:

Rabbit. Type: Polyclonal.

Epitope:

Peptide, DRLPKSDSEDGPRALNQLSC, corresponding to amino acid residues 493-511 of rH1 (accession number P15389).

Putative epitope location:

I/II intracellular loop.

Homology:

Mouse - identical; human - 17/19 amino acid residues identical.

Reactivity Confirmed:

Rat, human.

Applications:

Western Blotting:

Rat heart membranes (1:100 - 1:200).

Western blot analysis of Nav1.5 in rat heart membranes:   1. Anti-Nav1.5 antibody (#ASC-005), (1:200).    2. Anti-Nav1.5 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of Nav1.5 in Rat Cardiac Muscle

Immunohistochemical staining of Nav1.5 in rat myocardium paraffin-embedded section using Anti-Nav1.5 antibody (ASC-005), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxylin is used as the counterstain.

References:

1. Wu, L. et al. (2002) NeuroReport 13, 2547.

2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029