Anti-Nav1.4
- 貨號 Cat. No.
- ASC-020
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.4
| Product#: | ASC-020 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
Rat. |
| Epitope: |
Peptide EDKELKDNHILNHVG(C) corresponding to amino acid residues 877-891 of rat Nav1.4 (Accession P15390 ). |
 |
| Putative epitope location: |
Intracellular loop between II and III domains. |
| Homology: |
Mouse ?15/17 amino acid residues identical, human ?14/17 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat skeletal muscle lysate: 1. Anti-Nav1.4 antibody (#ASC-020), (1:200). 2. Anti-Nav1.4 antibody, preincubated with the control peptide antigen. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2005) Pharmacological Reviews 57, 397. |
| 8. Sasaki, R. et al. (1999) Arch. Neurol. 56,692. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally and tissue specific. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6
The isoform Nav1.4, is primarily expressed in skeletal muscle.7
Different missense mutations in the gene for the Nav1.4 are correlated with several muscular diseases such as, Paramyotonia congenital (PMC), PMC without cold paralysis, potassium-aggravating myotonia, and hyperkalemic periodic paralysis.8
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.4. The Anti- Nav1.4 (#ASC-020) can be used for Western blot analysis. It has been designed to recognize Nav1.4 from rat, human and mouse samples.