Anti-Nav1.3
- 貨號 Cat. No.
- ASC-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.3
| Product#: | ASC-004 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat Nav1.3 (Accession P08104). |
 |
| Putative epitope location: |
Intracellular, loop between I and II domains. |
| Homology: |
Mouse-12/14 amino acid residues are identical. |
| Reactivity Confirmed: |
Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of Nav1.3 in rat newborn brain membranes: 1. Anti-Nav1.3 antibody (#ASC-004), (1:200). 2. Anti-Nav1.3 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Nav 1.3 in dorsal root ganglia (DRG) of rat embryo |
 |
Immunohistochemical staining of rat embryo DRG frozen section of using Anti-Nav1.3 antibody (#ASC-004), (1:100). Nav1.3 is expressed in DRG embrionic cells (arrows). (Calibration bar = 50um) |
| Immunocytochemistry: |
 |
Immunocytochemical staining of Nav1.3 in rat Dorsal Root Ganglia (DRG) primary culture. A. Paraformaldehyde-fixed and permeabilized DRG cells were stained using Anti-Nav1.3 antibody (#ASC-004), (1:200) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merge image of panels A and B. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. |
| 8. Lai, J. et al. (2004) Annu. Rev. Pharmacol. Toxicol. 44, 371. |
| 9. Hains, C. B. et al (2003) J. Neurosci. 23, 8881. |
| 10. Cummins, T. R. et al (2001) J. Neurosci. 21, 5952. |
| 11. Shah, B. S. et al. (2001) J. Physiol. 534.3, 763. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6,7
Nav1.3 is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of Nav1.3 channel expression was described in injured neurons and injured spinal cord.9-11
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.3 channel. The Anti-Nav1.3 (#ASC-004) can be used for Western Blotting, Immunohistochemical and Immunocytochemical applications. It has been designed to recognize Nav1.3 from rat, human and mouse samples.