Anti-Nav1.2
- 貨號 Cat. No.
- ASC-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.2
| Product#: | ASC-002 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to residues 467-485 of rat Nav1.2 (Accession P04775). |
| Putative epitope location: |
Intracellular loop between I and II domains. |
| Homology: |
Human - identical. Homology with other isoforms: Nav1.3 - 12/15 residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-Nav1.2 antibody (#ASC-002), (1:200). 2. Anti-Nav1.2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Nav1.2 in Mouse Hippocampus |
 |
Immunohistochemical staining of NaV1.2 channel in mouse hippocampus using Anti-Nav1.2 antibody (#ASC-002 ). A, NaV1.2 channel (red) in dendrites of pyramidal neurons in the CA3 region. B, Staining of interneurons in the pyramidal layerwith mouse anti Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P). C, Confocal merge of panels A and B. |
| Immunoprecipitation: |
 | Immunoprecipitation of rat brain lysate: 1. Rat brain lysates 2. Rat brain lysates immunoprecipitated with Anti-Nav1.2 antibody (#ASC-002) (6 mg). 3. Rat brain lysates immunoprecipitated with pre-immune rabbit serum. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. |
| 8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6,7
Nav1.2 is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the Nav1.2 channel have been identified in different types of epilepsy.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.2 channel. The Anti-Nav1.2 (#ASC-002) can be used for Western Blotting, and Immunohistochemical applications. It has been designed to recognize Nav1.2 from rat, human and mouse samples.