Anti-Nav1.1
- 貨號 Cat. No.
- ASC-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Nav1.1
| Product#: | ASC-001 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)TAS EHSRE PSAAG RLSD, corresponding to amino acid residues 465-481 of rat Nav1.1 (Accession P04774) |
 |
| Putative epitope location: |
Intracellular loop between I and II domains. |
| Homology: |
Human and mouse - identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membranes: 1. Anti-Nav1.1 antibody (#ASC-001), (1:200). 2. Anti-Nav1.1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Nav1.1 channel in mouse cerebellum |
 |
Immunohistochemical staining of Nav1.1 channel in mouse cerebellum using Anti-Nav1.1 antibody (#ASC-001). A, The distribution of Nav1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B, Purkinje nerve cells are stained with mouse anti Parvalbumin (green). C, Confocal merge of Nav1.1 and Parvalbumin |
| Immunocytochemistry: |
 |
Immunocytochemical staining of Nav1.1 in rat Dorsal Root Ganglion (DRG). Paraformaldehyde-fixed and permeabilized DRG cells were stained using Anti-Nav1.1 antibody (#ASC-001), (1:200) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. |
| References: |
| 1. Wu, L. et al. (2002) NeuroReport 13, 2547. |
| 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. |
| 3. Fjell, J. et al. (2000) NeuroReport 11, 199. |
| 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. |
| 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. |
| 6. Isom, L.L. (2001) Neuroscientist 7, 42. |
| 7. Catterall, W.A. et al. (2003) Pharmacol Rev 55, 575. |
| 8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768. |
| 9. Rhodes, T.H.et al. (2008) Proc. Natl. Acad. Sci. 101, 11147. |
Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability.1 Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.9.4-5 The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7) and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9).2-3 Mammalian sodiumchannels are heterotrimers, composed of a central, pore-forming a subunit and two auxiliary b subunits. The expression of the a subunit isoforms is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain b1 through b4 subunits, whereas sodium channels in adult skeletal muscle have only the b1 subunit.6,7
Nav1.1 is a highly tetrodotoxin-sensitive channel and is broadly expressed in neurons.7
Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI).8,9
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat Nav1.1 channel. The Anti-Nav1.1 (#ASC-001) can be used for Western Blotting, Immunohistochemical Immunocytochemical applications. It has been designed to recognize Nav1.1 from rat, human and mouse samples.