Anti-MYL2 Antibody

Anti-MYL2 Antibody LS-B3584

MYL2 Mouse anti-Human Monoclonal (AT3B2) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Learn more

MYL2 Antibody Details

Target: Human MYL2

Host: Mouse

Reactivity: Human, Rat

Clonality: IgG2b,k Monoclonal [AT3B2] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1:1000 - 1:2000)

ELISA

Optimal dilution to be determined by the researcher.

 

MYL2 Antibody Specificity

Immunogen: MYL2 antibody was raised against recombinant human MYL2 (1-166aa) purified from E. coli

Specificity: human MYL2 (1-166aa)

MYL2 Antibody Packaging

Presentation: PBS, pH 7.4, 0.1% sodium azide

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.