Anti-MTHFD1 Antibody

Anti-MTHFD1 Antibody LS-B4110

MTHFD1 Goat anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4110 was determined to be 3.75 ug/ml. Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx. 110kD band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kD according to NP_005947.2). Recommended concentration: 1-3 ug/ml. Learn more

MTHFD1 Antibody Details

Target: Human MTHFD1

Host: Goat

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (3.75)

Western blot (WB) (1 - 3)

ELISA (1:1000)

Optimal dilution to be determined by the researcher.

 

MTHFD1 Antibody Specificity

Immunogen: MTHFD1 antibody was raised against synthetic peptide RGDLNDCFIPCTPK from an internal region of human MTHFD1 (NP_005947.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Orangutan (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Hamster (100%), Elephant (100%), Bat (100%), Horse (100%), Rabbit (100%), Pig (100%), Rat (93%), Dog (93%), Opossum (93%), Turkey (93%), Chicken (93%), Lizard (93%), Xenopus (93%), Bovine (86%), Stickleback (86%), Pufferfish (86%), Zebrafish (86%).

Epitope: Internal

Specificity: Human MTHFD1.

MTHFD1 Antibody Packaging

Presentation: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Storage: Store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.