Anti-mGluR1 (extracellular)-ATTO-488

Anti-mGluR1 (extracellular)-ATTO-488

Product#:

AGC-006-AG

Sizes:

50 µl

Host:

Rabbit. Type: Polyclonal.

Epitope:

Peptide (C)HEGVLNIDDYKIQMNK, corresponding to amino acid residues 501-516 of rat mGluR1 (Accession P23385).

Putative epitope location:

Extracellular, N-terminus.

Homology:

Mouse, human, dog- identical. Label: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Reactivity Confirmed:

Rat, mouse, human.

Applications:

Immunohistochemistry:

Expression of mGluR1 in rat cerebellum

Immunohistochemical staining of rat cerebellum frozen sections with Anti-mGluR1 (extracellular)-ATTO-488 antibody (#AGC-006-AG), (1:20).  Staining (green) appears in cerebellar Purkinje cells (arrows) and in the molecular layer.

Flow Cytometry:

Flow cytometry analysis of live intact human Jurkat T-cell leukemia cells. ___Jurkat cells unstained. ___Jurkat cells + Anti-mGluR1 (extracellular)-ATTO-488 antibody (#AGC-006-AG), (10mg).

L-Glutamate is the major excitatory neurotransmitter in the central nervous system.  It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

 

Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. These receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminal and intracellular C-terminal domains. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

 

mGluR1 and mGluR5 receptors signal through G_q/G₁₁ that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca²⁺. More downstream signaling pathways include activation of PKC and modulation of Ca²⁺ and K⁺ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.

 

mGluR1 is predominantly expressed in nervous tissues although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.

 

The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinsonî–¸ and Huntingtonî–¸ disease.

 

Alomone Labs offers a highly specific antibody directed against an extracellular epitope of the mGluR1 receptor. The Anti-mGluR1 (extracellular) antibody (#AGC-006) can be used in Western blot, immunocytochemical, immunohistochemical, flow cytometry and immunoprecipitation applications and recognizes mGluR1 from rat, mouse, and human samples.

 

Alomone Labs is pleased to offer a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The Anti-mGluR1 (extracellular)-ATTO-448 antibody (#AGC-006-AG) has been tested in immunohistochemical and flow cytometry applications and is specially suited for experiments requiring simultaneous labeling of different markers.