Anti-Melanocortin Receptor 4 (extracellular)
- 貨號 Cat. No.
- AMR-024
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Melanocortin Receptor 4 (extracellular)
| Product#: | AMR-024 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R (Accession P32245 ). |
 |
| Putative epitope location: |
| Extracellular, N terminal. |
| Homology: |
| Rat, mouse - 13/15 residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysates:
|
 | Western blot analysis of mouse brain lysates:
|
| Immunohistochemistry: |
 |
Expression of MC4R in mouse brain sections. Perfusion-fixed frozen sections were incubated with Anti-Melanocortin Receptor 4 (extracellular) antibody (#AMR-024), (1:100). MC4R expression (green fluorescence) appears in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 (blue) was used as a counterstain. |
| Immunocytochemistry: |
 |
Immunocytochemical staining of MC4R in a rat pituitary cell line. A.Live intact GH3 cells were stained with Anti-Melanocortin Receptor 4 (extracellular) antibody (#AMR-024), (1:50) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody (red staining). Nuclei were visualized using the cell-permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as A. |
Melanocortin Receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the a-, b-, and g-melanocyte-stimulating hormone (a-, b-, g-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3
One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1
All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is a-MSH = ACTH > b-MSH >> g-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3
MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope in the extracellular N-terminus of human MC4R. Anti-Melanocortin Receptor 4 (extracellular) antibody (#AMR-024) can be used in Western blot, immunohistochemical, and immunocytochemical applications, and recognizes MC4R from mouse and rat samples.