Anti-MED4 Antibody

Anti-MED4 Antibody LS-B3562

MED4 Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3562 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3562 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

MED4 Antibody Details

 

 

Target:

Human MED4

 

Host:

Rabbit

 

Reactivity:

Human, Mouse, Rat

 

Clonality:

IgG Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB)

 

Optimal dilution to be determined by the researcher.

 

MED4 Antibody Specificity

 

Immunogen:

MED4 antibody was raised against synthetic peptide from internal region of human MED4 (NP_054885.1, Q9NPJ6). Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

 

Epitope:

Internal

 

Specificity:

Human MED4

MED4 Antibody Packaging

 

Presentation:

Lyophilized from PBS with 2% sucrose

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.