Anti-MADCAM1 / MAdCAM-1 Antibody

Anti-MADCAM1 / MAdCAM-1 Antibody LS-B3316

MADCAM1 / MAdCAM-1 Mouse anti-Human Monoclonal (Low Endotoxin) (314G8) Antibody

An IHC-plusTM Antibody

 Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections. Western Blot: LS-C44686 detects a band of approximately 60kD. Reducing conditions required. Learn more

MADCAM1 / MAdCAM-1 Antibody Details

 

 

Target:

Human MADCAM1 / MAdCAM-1

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1 Monoclonal [314G8]

 

Purification:

Protein G Column

 

Format:

Low Endotoxin (Low Endotoxin)

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB)

 

Flow Cytometry (Flo) (1:10 - 1:100)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

MADCAM1 / MAdCAM-1 Antibody Specificity

 

Immunogen:

MADCAM1 antibody was raised against recombinant soluble MAdCAM-1-fc fusion protein.

 

Specificity:

Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7

MADCAM1 / MAdCAM-1 Antibody Packaging

 

Presentation:

PBS, pH 7.4.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.