Anti-m2

Anti-m2

Product#:

AMR-002

Sizes:

50 ml

0.2 ml

the Gbg subunit to the channel.^6,7 Stimulation of the m2 receptor by acetylcholine in the heart results in activation of the K_ir3.1/K_ir3.4 channels causing a slowing in heart beat.⁷

Host:

Rabbit.

Epitope:

GST fusion protein with a sequence VANQD PVSPS LVQGR IVKPN NNNMP SSDDG LEHNK IQNGK APRDP VTENC VQGEE KESSN DSTSV SAVAS NMRDD EITQD ENTVS TSLGH SKDEN SKQTC IRIGT KTPKS DSCTP TNTTV EVVGS SGQNG DE, corresponding to residues 225-356 of human m2 (Accession P08172).

Putative epitope location:

3rd intracellular loop.

Homology:

Chimpanzee, gorilla - identical; orangutan, pig, rat, mouse, dog - respectively, 130/132, 122/132, 118/132, 117/132, and 122/132 residues identical.

Applications:

Western Blotting:

Western blotting of rat brain membranes: 1.  Anti-m2 antibody, 1:200. 2.  Anti-m2 antibody, preincubated with the control fusion protein antigen.

Immunohistochemistry:

Rat brain sections and mouse parieto-temporal cortex sections.

Immunohistochemical distribution of Kir3.2 K+ channel and m2 receptor in mouse parieto-temporal cortex. Frozen sections (non-consecutive sections) of mouse parieto-temporal cortex were visualized using Anti-Kir3.2 antibody (#APC-006) (1:100) and Anti-m2 antibody (#AMR-002) (1:100). m2 staining was dense in layer IV, with fibers climbing to layers II-III. Kir3.2 staining was dense in layers IV and I. Overlapping expression of Kir3.2 channel and m2 is seen in cortical layers.

References:

1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333.

2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298.

3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411.

4. Van der Zee, E.A. and Luiten, P.G. (1999) Prog. Neurol. 58, 409.

5. Tata, A.M. et al. (1999) Brain Res. 824, 63.

6. Shapiro, M.S. et al. (1999) Proc. Natl. Acad. Sci. U.S.A.  96, 10899.

7. Jin, W. and Lu, Z. (1998) Biochemistry  37, 13291.

The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors  belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and are named m1-m5.^1-2



The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.^1-2

Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.¹ They also participate in learning and memory processing.^3-4

The m2 receptor is considered to be the predominant muscarinic receptor that is expressed in cardiac muscle.⁵

The m2 and m4 receptors mediate Ca²⁺ channel inhibition and K_ir3 K⁺ channel activation by directly binding