Anti-LIME1 / LIME Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3731 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3731 was determined to be 10 ug/ml. Learn more

LIME1 / LIME Antibody Details

 
 
 


Target:
Human LIME1 / LIME
 
Host:
Rabbit
 
Reactivity:
Human
 
Non-Reactivity:
Mouse
 
Clonality:
 Polyclonal
 
Purification:
Antiserum
 

Applications:
 
Immunohistochemistry - Paraffin (IHC-P) (10)
 
Western blot (WB)
 
Optimal dilution to be determined by the researcher.
 
LIME1 / LIME Antibody Specificity
 


Immunogen:
LIME1 / LIME antibody was raised against bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
 
Epitope:
aa141-295
 
Specificity:
Reacts with the cytoplasmic domain of LIME, a 31 kD Lck-interacting transmembrane adaptor expressed by T cells.


LIME1 / LIME Antibody Packaging
 


Presentation:
PBS, 15 mM sodium azide, approx., pH 7.4.
 
Storage:
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
 
Restrictions:
For research use only.