Anti-Kv4.1
- 貨號 Cat. No.
- APC-119
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kv4.1
| Product#: | APC-119 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
Peptide (C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of the human Kv4.1 (Accession Q9NSA2 ). |
 |
| Putative epitope location: |
| Intracellular, C-terminal domain. |
| Homology: |
Mouse, rat - 12/13 residues identical. |
| Reactivity Confirmed: |
| Human, rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain lysate:
|
 | Western blotting of human neuroblastoma cell line SH-SY5Y (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): 1, 2. Anti-KV4.1 (1:400) 3, 4. Anti-KV4.1, preincubated with the control peptide antigen. |
| References: |
| 1. Pak, M.D. et al. (1991) Proc. Natl. Acad. Sci. 88, 4386. |
| 2. Song, W.J. et al. (1998) J. Neurosci. 18, 3124. |
| 3. Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48. |
| 4. Birnbaum, S. et al. (2004) Physiol. Rev. 84, 803. |
Kv4.1 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: Kv4.2 and Kv4.3.
Kv4.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of Kv4.1 and Kv4.2.
The Kv4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of Kv4.1 and Kv4.2 subunits.
The biophysical properties of the Kv4.1 subunit can be modified by its association with auxiliary b subunits such as KChIP1 that increase Kv4.1 current densities and accelerates both the inactivation and the recovery time.
Kv4.1 is highly expressed in brain but is also expressed in peripheral tissues such as colon, heart and lung.
The pharmacology of Kv4.1 has been less well studied than that of the other Kv4 members. Kv4.1 is sensitive to the classical K+ channel blockers, 4-aminopyridine and tetraethylammonium. Recently HmTx1 a peptide blocker derived from the venom of the African tarantula Heteroscodra maculata has been shown to be a potent inhibitor of Kv4.1.
We have now developed a highly specific antibody directed against the intracellular C-terminus domain of the human Kv4.1 protein. The antibody will also recognize Kv4.1 from rat and mouse origins.