Anti-Kv3.3

Anti-K_v3.3

Product#:

APC-102

Sizes:

50 ml

0.2 ml

K_v3.3 is a member of the Shaw type family of voltage-gated K⁺ channels that includes four members (K_v3.1-3.4). 

K_v3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of  

action potentials and to facilitate repetitive high frequency firing.  

K_v3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium.  

In the brain its expression pattern largely overlaps that of the K_v3.1 channel suggesting that they may form functional  

heteromers. Indeed, mouse knockouts of both K_v3.1 and K_v3.3, but not either channel alone, display severe motor defects.

Host:

Rabbit.

Epitope:

Peptide KSPITPGSRGRYSRDRAC corresponding to  residues 701-718 of rat Kv3.3 (Accession number Q01956).

Putative epitope location:

Intracellular, C-Terminus.

Homology:

Human, mouse-identical. Antibody specificity:   Recognizes only splice variant Kv3.3 ?.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Rat brain membrane (1:200).

Western blotting of rat brain membranes: 1. Anti-Kv3.3 antibody (1:200).   2. Anti-Kv3.3 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Rat brain sections.

Immunohistochemical staining of Kv3.3 using Anti-Kv3.3 antibody (#APC-102).   Immunoreactive neurons in the reticular thalamic nucleus   detected with diaminobenzidine.   IH photograph kindly contributed by Jim Deuchars, Leeds, UK.

References:

1. Rudy, B. et al. (2001) Trends Neurosci. 24, 517.

2. Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.